Multicolor multiphoton microscopy based on a nanosecond supercontinuum laser source - Collection LabEx ∑_Lim
Article Dans Une Revue Journal of Biophotonics Année : 2016

Multicolor multiphoton microscopy based on a nanosecond supercontinuum laser source

Claire Lefort
Rodney Philip O'Connor
  • Fonction : Auteur
  • PersonId : 951224
Véronique V. Blanquet
Laetitia Magnol
  • Fonction : Auteur
Vincent Tombelaine
  • Fonction : Auteur
Philippe Lévêque
OSA
Vincent Couderc

Résumé

Multicolor multiphoton microscopy is experimentally demonstrated for the first time on a spectral bandwidth of excitation of 300 nm (full width half maximum) thanks to the implementation a nanosecond supercontinuum (SC) source compact and simple with a low repetition rate. The interest of such a wide spectral bandwidth, never demonstrated until now, is highlighted in vivo: images of glioma tumor cells stably expressing eGFP grafted on the brain of a mouse and its blood vessels network labelled with Texas Red® are obtained. These two fluorophores have a spectral bandwidth covering the whole 300 nm available. In parallel, a similar image quality is obtained on a sample of mouse muscle in vitro when excited with this nanosecond SC source or with a classical high rate, femtosecond and quasi monochromatic laser. This opens the way for (i) a simple and very complete biological characterization never performed to date with multiphoton processes, (ii) multiple means of contrast in nonlinear imaging allowed by the use of numerous fluorophores and (iii) other multiphoton processes like three-photon ones.
Fichier principal
Vignette du fichier
JOB_9-7.pdf (276.24 Ko) Télécharger le fichier
Origine Publication financée par une institution

Dates et versions

hal-01321985 , version 1 (01-10-2024)

Identifiants

Citer

Claire Lefort, Rodney Philip O'Connor, Véronique V. Blanquet, Laetitia Magnol, Hideaki Kano, et al.. Multicolor multiphoton microscopy based on a nanosecond supercontinuum laser source. Journal of Biophotonics, 2016, 9 (7), pp.709-714. ⟨10.1002/jbio.201500283⟩. ⟨hal-01321985⟩
95 Consultations
2 Téléchargements

Altmetric

Partager

More