Rod-shaped micropatterning enhances the electrophysiological maturation of cardiomyocytes derived from human induced pluripotent stem cells - Bordeaux Population Health
Article Dans Une Revue Stem Cell Reports Année : 2024

Rod-shaped micropatterning enhances the electrophysiological maturation of cardiomyocytes derived from human induced pluripotent stem cells

Zeina R Al Sayed
  • Fonction : Auteur
Charlene Jouve
  • Fonction : Auteur
Magali Seguret
  • Fonction : Auteur
Andrea Ruiz-Velasco
  • Fonction : Auteur
Celine Pereira
  • Fonction : Auteur
Jean-Sebastien Hulot
  • Fonction : Auteur

Résumé

Human induced pluripotent stem cell-derived cardiomyocytes (hiPSC-CMs) offer great potential for drug screening and disease modeling. However, hiPSC-CMs remain immature compared to the adult cardiac cells. Cardiomyocytes isolated from adult human hearts have a typical rod-shaped morphology. Here, we sought to develop a simple method to improve the architectural maturity of hiPSC-CMs by using a rod-shaped cell micropatterned substrate consisting of repeated rectangles (120 μm long × 30 μm wide) surrounded by a chemical cell repellent. The generated hiPSC-CMs exhibit numerous characteristics similar to adult human cardiomyocytes, including elongated cell shape, well-organized sarcomeres, and increased myofibril density. The improvement in structural properties correlates with the enrichment of late ventricular action potentials characterized by a more hyperpolarized resting membrane potential and an enhanced depolarization consistent with an increased sodium current density. The more mature hiPSC-CMs generated by this method may serve as a useful in vitro platform for characterizing cardiovascular disease.
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hal-04741122 , version 1 (17-10-2024)

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Zeina R Al Sayed, Charlene Jouve, Magali Seguret, Andrea Ruiz-Velasco, Celine Pereira, et al.. Rod-shaped micropatterning enhances the electrophysiological maturation of cardiomyocytes derived from human induced pluripotent stem cells. Stem Cell Reports, 2024, 19 (10), pp.1417-1431. ⟨10.1016/j.stemcr.2024.08.005⟩. ⟨hal-04741122⟩

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