Caveolae and Bin1 form ring-shaped platforms for T-tubule initiation - Plateforme d’immortalisation MyoLine – CRM
Article Dans Une Revue eLife Année : 2023

Caveolae and Bin1 form ring-shaped platforms for T-tubule initiation

Alexis Canette

Résumé

Excitation-contraction coupling requires a highly specialized membrane structure, the triad, composed of a plasma membrane invagination, the T-tubule, surrounded by two sarcoplasmic reticulum terminal cisternae. Although the precise mechanisms governing T-tubule biogenesis and triad formation remain largely unknown, studies have shown that caveolae participate in T-tubule formation and mutations of several of their constituents induce muscle weakness and myopathies. Here, we demonstrate that, at the plasma membrane, Bin1 and caveolae composed of caveolin-3 assemble into ring-like structures from which emerge tubes enriched in the dihydropyridine receptor. Bin1 expression lead to the formation of both rings and tubes and we show that Bin1 forms scaffolds on which caveolae accumulate to form the initial T-tubule. Cav3 deficiency caused by either gene silencing or pathogenic mutations results in defective ring formation and perturbed Bin1-mediated tubulation that may explain defective T-tubule organization in mature muscles. Our results uncover new pathophysiological mechanisms that may prove relevant to myopathies caused by Cav3 or Bin1 dysfunction.
Fichier principal
Vignette du fichier
84139.pdf (33.99 Mo) Télécharger le fichier
Origine Fichiers éditeurs autorisés sur une archive ouverte
licence

Dates et versions

hal-04094370 , version 1 (11-05-2023)

Licence

Identifiants

Citer

Eline Lemerle, Jeanne Lainé, Marion Benoist, Gilles Moulay, Anne Bigot, et al.. Caveolae and Bin1 form ring-shaped platforms for T-tubule initiation. eLife, 2023, 12, ⟨10.7554/eLife.84139⟩. ⟨hal-04094370⟩
552 Consultations
25 Téléchargements

Altmetric

Partager

More